huPBMC-B Mice

Alfa Cytology's huPBMC-B mice provide a specialized platform for studying human B cell biology and evaluating B cell-targeting therapies. This model achieves significant reconstitution of functional human B cells that mature into antibody-secreting plasma cells, enabling critical research in humoral immune responses, antibody production kinetics, and therapeutic interventions for B cell-related diseases. Our end-to-end services support your programs from model establishment to comprehensive functional analysis.

Introduction to huPBMC-B Mice

The huPBMC-B model is created by engrafting human PBMCs into immunodeficient NCG mice under conditions specifically optimized to enhance the survival, reconstitution, and functional maturation of human B cells.

• Robust B Cell Reconstitution: Hu-Immune™ platform demonstrates significant reconstruction of human CD19⁺ B cells in peripheral blood, spleen, and other lymphoid organs.
• Functional Antibody Secretion: The reconstituted B cells successfully differentiate into plasma cells capable of secreting functional human IgG, which is detectable in mouse plasma.
• Kinetic Readouts: Validated by ELISA, human IgG is detectable as early as Day 14, peaks around Day 22, and maintains stable secretion for over 35 days, providing quantitative pharmacodynamic data.

Fig.1 huPBMC-B: high-level B reconstruction.

Advantages of huPBMC-B Mice

High-Level B Cell Reconstitution Demonstrates significant human B cell reconstruction in the spleen and lymphoid organs of immunodeficient mice.

Functional B Cell Recovery & Antibody Secretion The reconstructed B cells possess full human IgG secretion capability. ELISA assays confirm sustained human IgG production in mouse plasma from Day 14 through Day 35, validating successful functional recovery.

Applications of huPBMC-B Mice

Case Study

Case 1: Robust B Cell Reconstitution in Spleen and Peripheral Blood

Overview

Validate the capacity of the huPBMC-B humanized model to reconstitute human B cells in spleen and peripheral blood and define a practical experimental window for B-cell–related studies.

Study Information

• Humanized Model: huPBMC-B on NCG strain.
• Engraftment: Human PBMCs from healthy donors.
• Analysis: Flow cytometry analysis of B cell levels in the spleen and peripheral blood at multiple time points post-engraftment.

Results

• Significant Splenic Reconstitution: Flow cytometry confirmed significant human B cell reconstitution in the spleens of engrafted mice compared to non-engrafted controls.
• Stable Peripheral Blood Engraftment: Peripheral blood monitoring demonstrated stable PBMC reconstitution over time, establishing a reliable window for B cell-related studies.

Fig.2 Robust human B cell reconstitution in huPBMC-B mice.

Case 2: Functional Human IgG Secretion in huPBMC-B Humanized NCG Model

Overview

Validate functional maturation of reconstituted human B cells into antibody-secreting plasma cells by quantifying human IgG kinetics in the huPBMC-B model.

Study Information

• Humanized Model: huPBMC-B on NCG strain
• Analysis: Longitudinal monitoring of human IgG concentration in mouse plasma via ELISA.

Results

• Human leukocyte reconstitution: G2 (engrafted) shows a time-dependent rise in peripheral hCD45⁺% to ~30–40% by Day 40–48; G1 remains near baseline.
• Functional secretion: human IgG is detectable by Day 21 in G2 and remains elevated vs. non-engrafted controls (G1) at Days 34 and 48, indicating ongoing antibody production.

Fig.3 Human leukocyte reconstitution and plasma IgG secretion in the huPBMC-B model.

The huPBMC-B model is the ideal preclinical platform for your B cell immunotherapy and humoral immunity research. Alfa Cytology's Hu-Immune™ platform delivers comprehensive services to support your program's success. Contact our scientific team now for customized solutions.

For research use only.